人翻译延伸因子eEF1A1促进肺癌细胞H1299对紫杉醇和阿霉素药物耐受的研究

Human elongation factor 1A1(eEF1A1) promotes drug tolerance in lung cancer cell line

摘要:

人翻译延伸因子eEF1A是翻译体系中的多功能蛋白质，其表达高低与细胞凋亡和细胞胁迫耐受相关.在已知eEF1A的多功能性和与细胞存活相关的基础上，本文进一步研究eEF1A的变体蛋白eEF1A1对癌细胞耐药性的影响.eEF1A1基因的蛋白质编码区经过克隆，并瞬时转染了人肺癌细胞H1299，在确定eEF1A1蛋白的胞内表达后，采用MTT法测定高表达eEF1A1的H1299细胞对抗癌药物紫杉醇和阿霉素的耐受.相对于对照细胞，eEF1A1的高表达可显著提高H1299细胞对阿霉素和紫杉醇的耐受性.通过耐药性测试揭示出eEF1A可能参与肿瘤发生的潜在功能.

Abstract:

Human translational elongation factor 1A （eEF1A）is a multi-functional protein besides its roles in protein translation system. The protein expressional level of eEF1A is related to cell viability in oxidative stress and apoptotic process. Based on the knowledge of multifunctional roles of eEF1A and its relation to cell viability, we studied the possible effect of the isoform eEF1A1 on the drug resistance in cancer cell lines. The eukaryotic expression vector containing eEF1A1 sequence was constructed and was transiently transfected into human lung cancer cell line H1299. After verifying the expression of FLAG -eEF1A1, MTT assay was utilized to measure the cell viability of transfected H1299 after treatment by two common anti-cancer drugs. H1299 transfected with eEF1A1 showed enhanced drug tolerance to both taxol and adriamycin. Lung cancer cell highly expressing EEF1A1 behave more tolerance to antineoplastic drugs.