This study aims to investigate the protective role of Cordyceps cicadae extracts(CCE)against H2O2induced oxidative stress in HeLa cells.HeLa cells were treated with CCE for 48h and 72 h to check cell viability.The SAβGaL activity and intracellular ROS was detected under the microscope after H2O2 treatment.The mRNA levels of CAT、SOD1、SOD2 and GPX1 were measured by real time PCR in HeLa cells after CCE treatment.Results show that CCE inhibited the growth of HeLa cells in a dosedependent manner.Pretreated HeLa cells with CCE (0.100mg/mL) showed decrease cellular senescence and level of ROS against H2O2induced oxidative stress.CCE can increase CAT and SOD1.Our results indicate that the CCE has protective effect against H2O2induced cellular senescence in HeLa cells.