大孔树脂纯化天麻多糖的工艺研究

Macroporous adsorption resin for the purification of polysaccharides from Gastrodia elata Bl

摘要:

本研究以炮制的干天麻为原料，水提醇沉法提取多糖，大孔吸附树脂纯化，比较了八种大孔树脂（AB-8、D101、LX-17、D301、NKA-9、S-8、LSD-001、ADS-7）对天麻多糖静态吸附解析效果，筛选出最佳纯化树脂，再研究最佳树脂纯化天麻多糖工艺参数.结果为：八种大孔吸附树脂中D101对天麻多糖的纯化效果最好.样品液浓度、温度、上样速度，洗脱用乙醇浓度、洗脱流速及洗脱体积等因素均对D101树脂吸附分离天麻多糖有影响.所得的最佳纯化工艺为：20 ℃是较适宜的吸附温度，上样速度1 BV/h，上样浓度4 mg/mL，进行吸附；吸附饱和平衡后，用解析液浓度60%乙醇，解析速率2 BV/h，解析液体积3 BV进行动态洗脱.通过该工艺天麻多糖的纯度提高到了65.7%，表明了大孔树脂D101对天麻多糖具有较好的纯化效果.

Abstract:

The Gastrodia elata polysaccharides were purified using a macroporous adsorption resins method. By comparing the Gastrodia elata polysaccharides separation effect of eight different types （AB-8、D101、LX-17、D301、NKA-9、S-8、LSD-001、ADS-7）of macroporous resin, D101 was selected as the ideal adsorbent as it had a strong adsorption ability and a high desorption rate. The optimal purification technology was as follows: loading rate1 BV/h, loading concentration 4 mg/mL, 60% ethanol as suitable elution solvent from the resin, the elution solvent volume 3 BV and the elution flow rate 2 BV/h. The purity of Gastrodia elata polysaccharide was increased from 15.6% to 65.7%, which indicated that macroporous resin D101 had better purification effect on Gastrodia elata polysaccharide.