丁酸钠对肾结石大鼠肠道菌群及炎症因子的影响

Effects of sodium butyrate on intestinal flora and inflammatory factors in rats with renal calcium oxalate stones

摘要:

为探讨丁酸钠对大鼠模型的肾草酸钙结晶数量、肠道菌群及炎症相关因子的影响，探究其干预肾草酸钙结石形成的可能机制，以乙二醇诱导大鼠成肾草酸钙结石模型，并予丁酸钠干预，对肾脏进行病理组织学检测，并采用qPCR检测肠道菌群特定菌属（Enterococcus、E. coli、Bacteroides fragilis、Bifidobacterium、Faecalibacterium prausnitzii、Lactobacillus、Roseburia spp.、Bacteroides、Firmicutes）数量，以及与炎症相关的脂多糖受体TLR4、促炎细胞因子IL-1β、NF-κB p50的mRNA相对表达量.结果表明，与肾草酸钙结石组相比，丁酸钠组的肾草酸钙结晶减少（P < 0.05）；Enterococcus、Bacteroides fragilis、E. coli相对丰度显著降低（P < 0.05），其它菌属的丰度无显著变化（P > 0.05）; IL-1β、NF-κB p50的mRNA表达量显著降低（P < 0.05）, TLR4的mRNA表达量无显著变化（P > 0.05）.因此，丁酸钠可以减少E. coli、Enterococcus、Bacteroides fragilis的丰度，抑制结肠炎症相关因子IL-1β、NF-κB p50的mRNA表达，减少大鼠肾草酸钙结石的形成.

Abstract:

To investigate the effects of sodium butyrate on the quantity of calcium oxalate stones, intestinal flora, and inflammationrelated factors in rats with renal calcium oxalate stones, and to explore the possible mechanism of its intervention in the formation of calcium oxalate stones, rats were induced with ethylene glycol into a calcium oxalate stone model, and treated with sodium butyrate. Kidney histological examination was performed, and qPCR was used to detect the relative abundance of specific intestinal flora (Enterococcus, E.coli, Bacteroides fragilis, Bifidobacterium, Faecalibacterium prausnitzii, Lactobacillus, Roseburia spp., Bacteroides, Firmicutes), and qPCR was also used to detect the mRNA relative expression levels of lipopolysaccharide receptor TLR4, proinflammatory cytokines IL-1β and NF-κB p50. Compared with the renal calcium oxalate stones group, the number of calcium oxalate stones in the sodium butyrate group was significantly reduced (P < 0.05). The abundance of Enterococcus, Bacteroides fragilis and E. coli were also significantly reduced (P < 0.05), but there were no significant changes in the abundance of other genera (P >0.05) . The mRNA expression levels of IL-1β and NF-κB p50 were significantly reduced (P < 0.05) too, but the mRNA expression level of TLR4 was not significantly changed (P > 0.05). Therefore, Sodium butyrate reduced the abundance of E.coli, Enterococcus and Bacteroides fragilis, inhibited the expression levels of colon inflammationrelated factors IL-1β and NF-κB p50, and reduced the number of calcium oxalate stones in rats.