蚕沙叶绿素不皂化物化学成分研究

Study on chemical constituents of Chlorophyll nonsaponifiable matter in Faeces Bombycis

摘要:

叶绿素不皂化物为蚕沙生产叶绿素过程中产量较大的副产物.为进一步提升蚕沙的综合利用价值，本文重点研究蚕沙叶绿素不皂化物中的化学成分.通过硅胶柱色谱、C18中压柱色谱、高压制备柱色谱等方法对叶绿素不皂化物进行分离纯化，并依据波谱数据和理化性质对得到化合物进行结构鉴定.此次共分离鉴定出11个化合物，分别为植物醇(1)、5-Glutinen-3-ol(2)、羽扇豆醇(3)、豆甾醇(4)、菜油甾醇(5)、β-谷甾醇(6)、α-生育酚(7)、γ-生育酚(8)、(2E,6E,10E,14E,18E,22E)3,7,11,15,19,23-hexamethyl-2,6,10,14,18,22-tetracosahexaen-1-ol(9)、(13,14,17,20R)-lanosta-7,24-diene-3-ol(10)、棕榈酸(11).从结果可知，蚕沙叶绿素不皂化物中富含甾醇类物质，且化合物2, 9, 10为首次从蚕沙中分离得到.

Abstract:

Chlorophyll non-saponifiable matter is a by-product with a large yield in the process of producing chlorophyll from Faeces Bombycis. To further enhance the comprehensive utilization value of Faeces Bombycis, this research focuses on the chemical constituents in chlorophyll non-saponifiable matter of Faeces Bombycis. Silica gelcolumn chromatography, C18 medium pressure column chromatography, high pressure preparative column chromatography was used for separation and purification. The structures of the compounds were identified by spectroscopic data and physicochemical properties. A total of 11 compounds were isolated and identified, which were phytol(1),5-glutinen-3-ol(2), lupeol(3), stigmasterol(4), campesterol(5), β-sitosterol(6), α-tocopherol(7), γ-tocoferol(8), (2E,6E,10E,14E,18E,22E)-3, 7, 11, 15, 19, 23-hexamethyl-2, 6, 10, 14, 18, 22-tetracosahexaen-1-ol(9), 13, 14, 17, 20R)-lanosta-7, 24-diene-3-ol(10), palmitic acid(11). According to the results, the main components of the non-saponifiable matter are rich in sterols, and compounds 2, 9 and 10 were isolated from Faeces Bombycis for the first time.