不同根肿病抗性油菜根系分泌物的代谢组研究

Metabolome studies of root exudates from different clubroot resistance Brassica napus

摘要:

为了探究寄主根系分泌物中对根肿菌休眠孢子萌发具有重要作用的代谢物，本研究首先测试了甘蓝型油菜不同根肿病抗性材料的根系分泌物对休眠孢子萌发的诱导能力，发现易感材料根系分泌物处理组的萌发率显著高于抗性材料处理组.进一步采用非靶向液相色谱质谱联用代谢物分析法检测了抗病材料和易感材料的根系分泌物，在负离子（ESI-）模式下鉴定出次级代谢产物1079个，在正离子（ESI+）模式下鉴定出次级代谢产物1257个，其中差异代谢物分别为662和722个. 选取部分差异较大的代谢物进行验证，结果表明，胸腺嘧啶，2′-脱氧尿苷，L-异亮氨酸，葫芦巴碱，磷酸吡哆醛，香豆素，D-来苏糖和穗花杉双黄酮等对休眠孢子萌发具有显著促进作用，继而影响根肿病的发生.

Abstract:

To investigate the metabolites in host root exudates that are important for the germination of Plasmodiophora brassicae resting spores, in this study, we first tested the induction ability of resting spores’ germination by root exudates from different clubroot resistant levels materials of Brassica napus, it was found that the germination rate induced by the root exudates of the susceptible materials was significantly higher than that of the resistant materials. Then, we analyzed the root exudates of two resistant and one susceptible material by non-targeted liquid chromatography-mass spectrometry metabolite profiling, non-redundant mass ions (1079 in ESI- mode and 1257 in ESI+ mode) were identified, of which 662 and 722 differential metabolites were screened in ESI- and ESI+ respectively. Some of the compounds with large differences were selected to be further verified. The results showed that Thymine, 2′-Deoxyuridine, L-isoleucine, Trigonelline, Pyridoxal 5′-phosphate, Coumarin, D-lyxose, and Amentoflavone had a significant promoting effect on the germination of resting spores and subsequently caused the development of clubroot.