高表达miR-122腺病毒载体构建与功能检测

Construction and identification of miR-122 expressing recombinant adenovirus

摘要:

旨在利用改造后的腺病毒表达载体pDC312-cmv，构建含有8个miR-122表达框的miR-122高表达重组载体p-8miR-122，通过RT-PCR、ELISA、Sourthern blot验证重组载体高表达miR-122的水平以及抗乙肝病毒活性的能力. 通过腺病毒的包装、扩增、纯化和滴度检测，获得高纯度的腺病毒并成功检测到miR-122高表达和抗乙肝病毒活性的能力.

Abstract:

To construct miR-122 overexpression Adenovirus vector，gene including eight miR-122 precursor fragments was inserted into recombinant construct pDC312-cmv. Abilities of the recombinant vector p-8miR-122 to express miR-122 level and anti-HBV activities were analysed by RT-PCR、ELISA、Sourthern blot. Through adenovirus packaging、amplification、purification and titer detection，the pDC312-cmv and p-8miR-122 adenovirus had a high purity，high miR-122 expression levels and significantly anti-HBV activities.