利用PCR-DGGE分析胆固醇结石中细菌群落结构

Analysis the bacterial community structure of human cholesterol gallstones by PCR-DGGE

摘要:

本研究运用聚合酶链式反应-变性梯度凝胶电泳（PCR-DGGE）方法对来自8名胆石病人的8份胆固醇结石和4份胆汁样品中细菌群落结构进行分析和比较研究。DGGE结果显示，胆固醇结石中细菌含有Enterococcus，Pseudomonas等13个属细菌，其中Pseudomonas检出率最高，Klebsiella和Bacillus两个属细菌含量较多；胆固醇结石中6个属细菌在胆汁中也检测到。不同病人之间胆固醇结石细菌群落结构高度相似，但胆汁相似度较低；胆固醇结石中细菌群落结构与胆汁细菌群落结构最高相似度仅为38%，胆汁中细菌种类虽与胆固醇结石有同源性，但细菌群落结构差异较大。因此，导致胆固醇结石形成的细菌种类有一定偏好性，不因人而异，临床上对这些细菌的控制将有利于抑制结石的生成。

Abstract:

In this study, the microbial community of cholesterol gallstones and bile were examined by polymerase chain reaction-denaturing gradient gel electrophoresis (DGGE). The DGGE results showed that there are 13 genera in cholesterol gallstones. Pseudomonas was the dominant bacterium; moreover, Klebsiella and Bacillus had greater abundance than other genera. There were 6 genera disclosed in both of cholesterol gallstones and bile. In 4 patients, the bacteria community of cholesterol gallstones had higher similarity than from bile. The bacterial species that can cause the formation of cholesterol gallstones may be similar between different patients. The highest similarity coefficient between cholesterol gallstones and bile was only 38%, indicating the bacterial community of cholesterol gallstone was different from bile.