麻疯树适生区域土壤DNA提取及多重PCR验证

Extraction of Total DNAs from Soil Samples of Jatropha curcas L. Available Regions and Validation by Multiplex-PCR Method

摘要:

文章运用了4种土壤总DNA提取方法提取四川西昌麻疯树适生区土壤样品的DNA，并通过提取效果之间的比较发现改进后的方法1和方法4提取效果显著优于方法2和方法3。不仅如此，多重PCR扩增结果显示方法1和方法4提取的土壤总DNA扩增的真菌DNA产量所占比例增加，说明所得DNA更具有代表性。然而仅有方法4能成功提取海南及云南两地麻疯树适生地区的土壤总DNA，但提取的DNA纯度和产量过低，说明还需进一步进行优化。

Abstract:

In this paper, four kinds of soil total DNA extracting methods are applied to soil sample from Xichang, Sichuan, an available region for Jatropha curcas L. growth and through comparison of extracting effects, we find improved methods, method 1 and method 4, show better extracting results than method 2 and method 3. Moreover, the results of multiplex-PCR amplification show the soil total DNA extracted by method 1 and method 4 can obtain higher ratio of fungi DNA, suggesting the DNA extracted by this two methods is more representative. However, only method 4 can successfully extract total DNA of the soil samples from Hainan Province and Yunnan Province, the low DNA purity and yield meaning further modification is needed.