Abstract:Transformation of Chlamydomonas reinhardtii strain CC-400 with the plasmid pSP108 was carried out by glass beads method and twenty four transgenic monoclonal algae strains were successfully achieved by resistance selection and PCR identification. Two pairs of primers were designed at the middle and end part of the coding sequence of the exogenous gene ble. The transcription level of the transgenic monoclonal algae strains were detected by fluorescence quantitative PCR. The results showed that the transcription level of the exogenous gene ble in different transgenic monoclonal algae strains have obvious difference. In addition, the transcription level of exogenous gene ble in the same transgenic monoclonal algae strain was different though using two pairs of primers. That showed the copies, length and integrity of exogenous gene integrated into the genome were uncertainty.