川滇琼地区麻疯树种子核糖体失活蛋白资源调查

A Resources Investigation on the Content of Curcin in Jatropha Seed Endosperm Collected from Six Location in Sichuan, Yunnan and Hainan Province

摘要:

在对麻疯树核糖体失活蛋白的开发利用方面，天然curcin与原核表达的curcin相比在蛋白质折叠、修饰方面具有优势，对天然curcin进一步的深入研究和推广应用需要有充足的资源保障。本研究对西昌、攀枝花、仁里、海口、三亚、东方六地的麻疯树种子进行资源调查研究，结果显示六个地区麻疯树种子胚乳curcin含量均值为1.8770-2.5602 mg/g，由高到低依次为西昌、三亚、海口、仁里、攀枝花、东方，且地区间差异较大。各地区内部种子胚乳curcin含量数据分析显示，仁里地区种子胚乳curcin含量变异系数最大，为14.6189；海口地区的变异系数最小，为7.1876。麻疯树种子性状相关性分析结果显示胚乳curcin含量与百粒重、出仁率值在0.01水平上没有相关性，与含油率相关性不显著，与可溶性蛋白含量在0.01水平上低度负相关。综合各项指标认为西昌地区的麻疯树种子curcin含量较高，可作为现阶段curcin应用的材料来源之一。

Abstract:

The ribosome inactivating protein (curcin) expressed in Jatropha curcas L provides advantages, comparing to prokaryotic expression systems, on the study of protein folding and modification for better development and application of the protein. Therefore, the preservation and investigation of the plant resource for future research and application of the protein are necessary. In our study, a resources investigation was implemented, with the seeds of Jatropha curcas L collected from 6 individual locations (Xichang, Panzhihua, Haikou, Sanya, Renli and Dongfang) in Sichuan, Yunnan and Hainan province. The results showed that the average content of curcin in Jatropha curcas seed endosperm from all six locations is 1.8770-2.5602 mg/g, in descending order of Xichang, Sanya, Haikou, Renli, Panzhihua, Dongfang with relatively large differences between each locations. As illustrated in the data analysis, the seeds collected from Renli provided the highest coefficient of variation (14.6189) and the lowest (7.1876) was recorded in Haikou. The traits correlation of the seeds showed that the content of curcin in endosperm was not associated with hundred-seed-weight and kernel percentage and had weak relevance with the oil from whole seed, but a slight negative correlation was found in the analysis of the relevance of the content of curcin and soluble protein in endosperm (α=0.01). Based on these data, we concluded that the seeds in Xichang with a higher curcin expression could be used as a source of material in the research and application in curcin at this stage.