郁金茎叶提取物抑杀植物病原真菌活性研究与GC/MS分析

Antifugal activity and GC/MS analysis of extracts from Curcuma phaeocaulis stems and leaves against plant pathogenic fungi

摘要:

为探究郁金茎叶提取物对植物病原真菌的抑制效果及活性成分，以95%乙醇提取郁金茎叶制得浸膏依次用正己烷、乙酸乙酯和甲醇萃取，比较研究其对植物病原真菌的抑菌活性。采用GC/MS分析郁金茎叶正己烷萃取物和醇提物的化学组分，结果表明：醇提物与正己烷萃取物对小麦赤霉菌、油菜菌核菌、山葵墨入菌和辣椒灰霉菌均有较强抑制效果；正己烷萃取得率最高(64.68%)、抑菌活性最高；GC/MS分析结果显示正己烷萃取物中的11种成分含量增加，23种成分含量降低；正己烷萃取物中莪术烯醇(curcumenol)的含量最高(24.46%)，其次是棕榈酸甲酯(hexadecanoic acid, methyl ester; 13.84%)。GC/MS结果表明醇提物含28种化合物，正己烷萃取物含23种化合物。

Abstract:

To not only compare the in vitro antifungal activities against phytopathogens between ethanol and n-Hexane extracts of the stems and leaves from C. phaeocaulis, but also the differences of their active chemical components. The ethanol extractum of the stems and leaves from C. phaeocaulis had been prepared, then it was not only extracted by n-Hexane, ethylacetate, and methanol, but also the ethanol and n-Hexane extracts from stems and leaves were chemically analyzed by GC/MS. Results indicated that both the ethanol and n-Hexane extract showed well antifungal activity against pathogenic fungi including Fusarium graminearum, Phoma wasabiae, Sclerotinia sclerotiorum and Botrytis cinerea. Relative antifungal activities of three extracts, were compared and the yield of n-Hexane was 64.68%, higher than those of others while its antifungal activity was also the highest. The GC/MS results showed that there were 11 components increased but 23 components decreased in concentrations, and the highest components were curcumenol (24.46%) and hexadecanoic acid, methyl ester (13.84%) in n-Hexane extract. There were 28 components in ethanol extractum and 23 ones in n-Hexane extractum identified by GC/MS analysis.