过量表达BnTR1的油菜对多种逆境的综合抗性分析

Analysis of comprehensive tolerance to abiotic stresses in Brassica nupus over-expressing BnTR1

摘要:

油菜BnTR1是一个定位于质膜上的E3连接酶，前期的研究表明它赋予油菜、水稻和大肠杆菌对热胁迫的耐受性。为了研究过量表达BnTR1的油菜对多种逆境的综合抗逆特性，本研究设计了多种胁迫条件，观察并分析了这些油菜的综合抗逆表现。结果表明与对照材料相比，转基因油菜对双氧水胁迫、盐和甘露醇模拟干旱胁迫表现出较强的抗性，并且在盐胁迫下，转基因油菜的SOD、POD、和CAT三种抗氧化酶活性高于对照材料。这些结果证实过量的BnTR1使得油菜对多种胁迫表现出了较强的抗性，为深入研究BnTR1的功能奠定了基础，也为将这些油菜用作抗逆油菜新品种培育提供了原始材料。

Abstract:

BnTR1 is an E3 ligase localized in the plasma membrane, and the previous studies indicated that it played a key role in heat stress response in Brassica nupus, rice and E. coli. In order to investigate the comprehensive resistance characteristic of B.napus over-expressing BnTR1 lines, this research designed a variety of stress conditions to observe and analysis their phenotype. Results showed that compared with the control, the transgenic plant showed a better resistance to hydrogen peroxide, salt, and mannitol. In addition, the activity of superoxide dismutase(SOD),peroxidas (POD) and catalase(CAT) in transgenic plant was higher than the control under salt stress. These results confirmed that the over expression of BnTR1 enhanced the tolerance of Brassica nupus against a variety of stress, which laid a foundation for further research of the function of BnTR1, also provided original materials for breeding new varieties.