见血青总生物碱凝血活性的研究

Study on coagulation activity of total alkaloids in Liparis nervosa (Thunb.) Lindl.

摘要:

用超声波辅助提取见血青总生物碱(TALN)，配置成10mg/ml见血青总生物碱药液（TASLN）. 实验以生理盐水（NS）为空白对照、云南白药做阳性对照，体内凝血实验以小白鼠为研究对象测定其出血时间和出血量；局部创面止血实验以兔子为实验对象，记录背部创伤止血时间；体外凝血实验选用抗凝兔血，分别测定凝血时间、凝血酶原时间以及复钙时间. 结果显示：TALN的体内和体外凝血实验与空白对照组相比，明显缩短了老鼠出血时间（212.95s）和出血量（42.5格）、家兔的凝血时间（凝血板法时间为192.23s、试管法时间为245.48s）、凝血酶原时间（PT）（12.22s）及血浆复钙时间（RT）（180.78s）；局部创面止血实验结中，TASLN组平均止血时间为81.50s，相对于NS组125.20s明显缩短且与云南白药组75.20s的结果差异不显著。结论：TALN具有良好的凝血活性.

Abstract:

We configured a solution (TASLN) of 10 mg/mL total alkaloids of Liparis nervosa (TALN), which was extracted by Ultrasonic-assisted method. Normal saline (NS) was used as blank control and Yunan Baiyao as control in experience. For coagulation in vivo, the object was mice and we measured the bleeding time and the amount of bleeding. For coagulation in vitro, the object was rabbits. We recorded the hemostasis time of wound. the rabbits' anticoagulant blood was used to measure the coagulation time, prothrombin time (PT), Recalcification time (RT), respectively. The results showed that the time and the amount of mice bleeding was 212.95 s and 42.5 square. The coagulation time was 192.23s by blood congeals method and 245.48 s by tube method, PT and RT of rabbits' blood of TALN was 12.22 s and 180.78 s, all the times were shorter than the blank control. The average hemostasis time of total alkaloids solution of Liparis nervosa (81.50 s) was obviously shorter than that of NS (125.20 s), but showed no significant difference with that of Yunnan Baiyao (75.20 s). The TALN shows obvious coagulation activity.