拟南芥CARK3与RCAR12相互作用的研究

An Interaction of ABA Receptor RCAR12 with CARK3 in Arabidopsis thaliana

摘要:

植物激素ABA是植物应对非生物胁迫的响应因子，广泛参与了植物生长发育的调控。ABA结合其受体后抑制PP2Cs的磷酸酶活性，释放SnRK2s，从而启动ABA信号转导途径。本文采用酵母双杂交系统，GST-pull down技术，双分子荧光互补实验研究了激酶CARK3与ABA受体RCAR12在体外、体内的相互作用。体内外实验结果表明，CARK3与RCAR12存在明显的相互作用，CARK3可能通过直接磷酸化ABA受体RCAR12来正调控ABA信号途径的生理应答。本研究对进一步揭示ABA信号通路核心元件（RCARs/PYR1/PYLs）的磷酸化调控网络奠定了基础。

Abstract:

The phytohormone abscisic acid (ABA) is crucial for plant development and response to abiotic stress, including drought. ABA perceived by the ABA receptors, PYR/PYL/RCAR, which bound to ABA, and then recruit Protein phosphatase 2C (PP2C) to inhibit the PP2C acticity, thereby activating SNF1-RELATED PROTEIN KINASE2 (SnRK2s). Here, CARK3 and RCAR12 interact in yeast two-hybrid system and GST-pull down in vitro. Bimolecular fluorescence complementation (BiFC) was used to illuminate the interaction between CARK3 and RCAR12 in Nicotiana benthamiana in vivo. We could confer that CARK3 indeed interacts with RCAR12 in present study and the phosphorylation modulates the physiological response of the core ABA signaling pathway. Our results provide the foundation for further understanding of the regulatory network of phosphorylation of ABA signaling pathways core components.