甘蓝型油菜矮化突变体的茎秆发育转录组分析

Transcriptome analysis on stem development of a Brassica napus L. dwarf mutant

摘要:

甘蓝型油菜矮化突变体“NDF-1”是从高秆油菜“3529”理化诱变群体中分离的矮化突变体，它的株高只有高秆亲本的1/3，是一个优良的甘蓝型油菜矮化资源。本文应用RNA-Seq测序技术分析了“NDF-1”茎开始伸长的抽薹期和花期转录组，为深入了解油菜茎秆发育机理提供基因组层面的参考。抽薹期和花期的测序数据通过筛选得到了4.55亿条质量合格的clean reads。经过对高质量序列的组装注释，筛选得到“NDF-1”和“3529”间的2147条差异表达基因(DEGs)。选择其中的20个差异表达基因进行了荧光定量PCR验证。通过GO和KEGG分析，发现“NDF-1”的矮化与赤霉素、生长素和油菜素内酯相关，细胞伸长异常和细胞壁的不正常形成也影响“NDF-1”的茎秆发育。从差异基因中筛选出与植物矮化性状相关的基因，为利用矮化基因改善性状和矮化机理的研究和提供了更多信息

Abstract:

'NDF-1' is a Brassica napus L. dwarf mutant, which was derived from physical and chemical mutagenesis of a high stemmed line '3529 '，possesses a stable dwarf phenotype of approximately one third the height of high stalk parent '3529'. It is an excellent resource dwarf Brassica napus L. RNA-Seq based transcriptome analysis on stem elongation of the dwarf mutant from Bolting Stage (BS) and Florescence Stage (FS), which was conducted to provide a reference for the genome level in order to understand the mechanism of rapeseed stem development. A total of 455 million clean reads were obtained from two different stages include Bolting Stage (BS) and Florescence Stage (FS). Then 2,147 differentially expressed genes (DEGs) were found which detected between 'NDF-1' and '3529' after assembled them. Furthermore, twenty differentially expressed genes were selected for RT-PCR validation. With the GO and KEGG pathway analysis, 'NDF-1' stems elongation most regulated by the gibberellin、auxin and brassinosteroids, cell wall formation or cells Elongation is not normal but also play an important role in 'NDF-1' dwarf. Screening genes associated with plant dwarfing traits from DEGs, which provide a reference for further use of these genes on traits improvement and dwarfing mechanism research.