藏红花侧芽中L-瓜氨酸的提取与测定

Extraction and determination of L-Citrulline in the lateral bud of saffron

摘要:

对藏红花（Crocus sativus L.）侧芽中的L瓜氨酸进行定性定量分析，并对提取方法进行优化.样品采用超声波法提取，2,4-二硝基氟苯（FDNB）柱前衍生，通过超高效液相色谱进行分析.实验结果表明L-瓜氨酸在0.98~62.50μg/mL（R2= 0.9998）范围内线性关系良好，检测限（S/N=3）0.2440ng/mL，样品平均回收率为101.15% (n=6,RSD=1.00% ).藏红花侧芽中含有丰富的L瓜氨酸，提取条件经优化后，测得藏红花侧芽中L瓜氨酸的含量约为26.69 mg/g.

Abstract:

L-citrulline in the lateral bud of saffron was quantitatively analyzed for the first time and the extraction method was optimized. After ultrasonic extraction, the samples were derived with 2,4-dinitrofluorobenzene (FDNB) and analyzed by Ultra High Performance Liquid Chromatography. The results showed that a good linear relationship was obtained for L-citrulline in the concentration range of 0.98~62.50 μg/mL with R2 = 0.9998,the detection limit of L-citrulline was 0.2440ng/mL（S/N=3）, and the average recovery was 101.15% ( n=6, RSD=1.00% ). After the extraction method was optimized,the content of L-citrulline in the lateral bud of saffron was determined to be about 26.69 mg/g.