Abstract:In order to investigate whether ABA receptors participate in ABA homeostatic regulation mediated by UGT71B6 and analyze the specific mechanisms of UGT71B6's involvement in plant stress responses, we analyzed the interaction between RCARs and UGT71B6 by yeast two-hybrid assay, BIFC, and GST-pull down assay. The results indicated that UGT71B6 interacted with RCAR12/13 in vivo and in vitro. Thus, it is likely that the mechanism of UGT71B6 participating in plant abiotic responses is related to its interaction with ABA RCARs. The experimental results also provide some references for the role studying of ABA receptors in the ABA homeostasis regulation process.