RBM5蛋白的RNA识别区在肿瘤细胞周期调控中作用的研究

Research on the role of RNA recognition motif domains of RBM5 protein in the regulation of tumor cell cycle

摘要:

为分析RNA识别区（RNA recognition motif，RRM）在RBM5蛋白调控肿瘤细胞周期中的作用，本研究利用低表达RBM5的A549细胞系构建了A549/Vector、A549/RBM5WT、A549/RBM5△RRM三种稳定细胞株，通过流式细胞术检测，发现仅有A549/RBM5WT能显著阻滞细胞于G1期，说明RRM区是RBM5抑制细胞周期进程必不可少的区域.利用Western Blot检测细胞中cyclin A与PhosphoRb（ser 795）的蛋白水平差异，证明了RBM5蛋白的RRM能够参与其介导的pRb去磷酸化以及抑制cyclin A表达，从而抑制细胞周期进程.

Abstract:

In order to analyze the role of RNA recognition region (RRM) in the regulation of tumor cell cycle by RBM5 protein, three stable cell lines A549/Vector, A549/RBM5WT, A549/RBM5△RRM were constructed by using A549 cell line with low expression of RBM5. The phase of cell cycle analysis by Flow cytometry showed that only A549/RBM5WT was arrested in G1 phase, indicating that the RRM region was essential for RBM5 to inhibit cell cycle progression. Using Western Blot to detect the difference between cyclin A and PhosphoRb (ser 795), it was proved that RRM of RBM5 protein could participate in pRb dephosphorylation mediated by RBM5 and inhibit cyclin A expression, thus inhibiting cell cycle progression.