一种新型DNA纳米结构的构建与结晶

Construction and crystallization of a novel DNA nanostructure

摘要:

DNA 三角形纳米结构的拐角是由DNA同源重组中的Holliday基序构成的.为进一步研究DNA三角形纳米结构，对其拐角截断并引入黏性末端到其基序上，使其能够自组装成为一种新型DNA纳米结构．对该DNA纳米结构进行凝胶电泳分析，发现其迁移速率要比DNA三角形迁移速率小．在这种没有扭曲张力的情况下，该基序只是自组装形成一个二聚体结构，而不是三聚体结构（DNA三角形）．对该新型DNA纳米结构进行结晶，并合成得到了硒代核酸，同时也得到了较高质量的硒代单晶，以帮助相位测定．希望对其晶体结构进行测定研究，以便发现该新型DNA纳米二聚体结构和该Holliday基序组装的三维结构，从而更深入地认识DNA纳米材料的组装规律.

Abstract:

In the DNA triangle nanostructure, its corner is composed of Holliday junction in DNA homologous recombination.To further investigate it，the DNA triangle was modified by introducing the sticky end to the holiday junction motif to make it selfassemble into a DNA nanostructure.The gel electrophoresis analysis showed that the migration rate of this new DNA nanostructure was faster than that of the DNA triangle. Without the distortion，the motif assembled to form a dimer structure instead of a trimer triangle. The DNA nanostructure was crystallized and modified with selenium to obtain high quality crystals. The DNA nanostructure and the structure of the Holliday motif under non-twisting tension will be further studied and determined in order to offer more insights into DNA nanomaterial interaction and self-assembly.