利用宏基因组测序技术对大熊猫阴道菌群的研究

Study on the vaginal flora of giant pandas by using metagenomics

摘要:

为了阐明大熊猫阴道微生物组成，为合理的进行大熊猫阴道感染治疗提供科学依据，本研究利用宏基因组测序技术对不同地区（组别：WLQ、BFX、DJY）和不同年龄（组别：A1、A2、A3）的14个大熊猫阴道分泌物样品进行微生物菌群分析.结果表明：在物种注释上，占主导地位的微生物为变形菌门、担子菌门、厚壁菌门和放线菌门；在功能注释上，注释为碳水化合物代谢、氨基酸代谢的基因是样品中含有微生物数量最多的功能基因；在抗性基因注释上，变形菌门比厚壁菌门和拟杆菌门更易携带耐药基因，且沙门氏菌耐药基因APH3-lb分布存在于所有样品中.此外，不同年龄组大熊猫阴道菌群差异显著；DJY组衣原体比例最高.耐药基因APH3-lb和衣原体对大熊猫繁育存在一定的威胁，需要持续关注.

Abstract:

In order to clarify the microbial composition of the vagina of giant panda, which will provide a scientific basis for reasonable treatment of vaginal bacterial infections in giant pandas, the microbiological analysis of 14 vaginal secretion samples from different regions (groups: WLQ, BFX, DJY) and different ages (groups: A1, A2, A3) was performed by using metagenomic sequencing technology. The results showed that in the species annotation, the dominant phyla in these samples were Proteobacteria, Basidiomyta, Firmicutes and Actinobacteria. At the genus level, Pseudomonas was the most abundant, followed by Streptococcus, Psychrobacter and Proteus. In functional anotation, the gene annotated as carbohydrate metabolism and amino acid metabolism are the functional genes with the highest number of microorganisms in the samples. In terms of resistance gene annotation, Proteobacteria are more likely to carry drug-resistance genes than the Firmicutes and Bacteroidetes. The Salmonella resistance gene APH3-lb is present in all samples. Besides, the vaginal flora of giant pandas was significantly different in different age groups, and the DJY group has the highest proportion of chlamydia. The resistance gene APH3-lb and chlamydia pose a certain threat to the breeding of giant pandas, which need continuous attention.