响应面法优化黄精叶多糖水提工艺

Optimization of water extraction process for polysaccharides from leaves of polygonatum by response surface methodology

摘要:

为建立与优化黄精叶多糖提取工艺， 采用水提法提取黄精叶多糖，利用蒽酮硫酸法测定多糖含量. 以多糖提取率及出膏率为试验指标，在单因素试验基础上，通过三因素三水平Box-Behnken响应面法（RSM）优化黄精叶中多糖的提取条件. 结果显示黄精叶多糖最佳提取工艺为：提取温度75 ℃，液料比15∶1，提取时间2.5 h. 在此条件下，药材的出膏率为(47.65±0.16)%、多糖得率为(7.21±0.073)%. 该水提工艺条件稳定可行，多糖得率较高.

Abstract:

To establish and optimization the extraction process of polysaccharide from Polygonatum leaves, the polysaccharide was extracted by the water extraction method, and the content of polysaccharide was determined by the anthronesulfuric acid method. Based on the yield rate of polysaccharide and the extraction ratio, the three-factor and three-level Box-Behnken response surface methodology(RSM) was used to optimize the extraction process of polysaccharide from Polygonatum leaves by single factor test. The best extraction process of polysaccharide from Polygonatum leaves was: extraction temperature 75 ℃, the ratio of liquid-material 15∶1, extraction time 2.5 h. Under this condition, the extraction ratio was (47.65±0.16) %, and the polysaccharide yield rate was (7.21±0.073) %. The water extraction process of polysaccharide from polygonatum leaves was stable and feasible, and also has a high yield rate.