引用本文格式: 蒋海宾,杜宇,范小雪,王杰,祝智威,范元婵,熊翠玲,付中民,徐国钧,陈大福,郭睿. 蜜蜂球囊菌菌丝和孢子的比较转录组分析 [J]. 四川大学学报: 自然科学版, 2020, 57: 1177~1185.
 
蜜蜂球囊菌菌丝和孢子的比较转录组分析
Comparative transcriptome analysis of Ascosphaera apis mycelium and spore
摘要点击 195  全文点击 86  投稿时间:2020-01-29  修订日期:2020-05-21
查看全文  查看/发表评论  下载PDF阅读器
DOI编号   
中文关键词   蜜蜂球囊菌  菌丝  孢子  信号通路  差异表达基因
英文关键词   Ascosphaera apis  Mycelium  Spore  Signaling pathway  Differentially expressed gene
基金项目   国家自然科学基金(31702190);国家现代农业产业技术体系建设专项资金(CARS 44 KXJ7);福建省自然科学基金(2018J05042);福建省教育厅中青年教师教育科研项目(JAT170158);福建农林大学杰出青年科研人才计划项目(xjq201814);福建农林大学硕士生导师团队项目;福建农林大学优秀硕士学位论文资助基金(杜宇)
作者单位E-mail
蒋海宾 福建农林大学动物科学学院(蜂学学院)  
杜宇 福建农林大学动物科学学院(蜂学学院)  
范小雪 福建农林大学动物科学学院(蜂学学院)  
王杰 福建农林大学动物科学学院(蜂学学院)  
祝智威 福建农林大学动物科学学院(蜂学学院)  
范元婵 福建农林大学动物科学学院(蜂学学院)  
熊翠玲 福建农林大学动物科学学院(蜂学学院)  
付中民 福建农林大学动物科学学院(蜂学学院)
福建农林大学蜂产品加工与应用教育部工程研究中心 
 
徐国钧 福建农林大学动物科学学院(蜂学学院)
福建农林大学蜂产品加工与应用教育部工程研究中心 
 
陈大福 福建农林大学动物科学学院(蜂学学院)
福建农林大学蜂产品加工与应用教育部工程研究中心 
 
郭睿 福建农林大学动物科学学院(蜂学学院)
福建农林大学蜂产品加工与应用教育部工程研究中心 
fafu_ruiguo@126.com 
Author NameAffiliationE-mail
JIANG Hai-Bin College of Animal Sciences (College of Bee Science), Fujian Agriculture and Forestry University  
DU Yu College of Animal Sciences (College of Bee Science), Fujian Agriculture and Forestry University  
FAN Xiao-Xue College of Animal Sciences (College of Bee Science), Fujian Agriculture and Forestry University  
WANG Jie College of Animal Sciences (College of Bee Science), Fujian Agriculture and Forestry University  
ZHU Zhi-Wei College of Animal Sciences (College of Bee Science), Fujian Agriculture and Forestry University  
FAN Yuan-Chan College of Animal Sciences (College of Bee Science), Fujian Agriculture and Forestry University  
XIONG Cui-Ling College of Animal Sciences (College of Bee Science), Fujian Agriculture and Forestry University  
FU Zhong-Min College of Animal Sciences (College of Bee Science), Fujian Agriculture and Forestry University
Engineering Research Center of Processing and Application of Bee Products of Ministry of Education 
 
XU Guo-Jun College of Animal Sciences (College of Bee Science), Fujian Agriculture and Forestry University
Engineering Research Center of Processing and Application of Bee Products of Ministry of Education 
 
CHEN Da-Fu College of Animal Sciences (College of Bee Science), Fujian Agriculture and Forestry University
Engineering Research Center of Processing and Application of Bee Products of Ministry of Education 
 
GUO Rui College of Animal Sciences (College of Bee Science), Fujian Agriculture and Forestry University
Engineering Research Center of Processing and Application of Bee Products of Ministry of Education 
fafu_ruiguo@126.com 
中文摘要
    为探究实验室条件下纯培养的蜜蜂球囊菌(Ascosphaera apis)菌丝(AaM)和孢子(AaM)的共有基因、特有基因以及差异表达基因(differentially expressed gene, DEG)与真菌的生长、发育、生殖和致病性的相关性,利用RNA-seq技术和生物信息学方法对AaM和AaS进行比较转录组分析,并结合实时荧光定量PCR验证转录组数据的可靠性.分析结果显示AaM和AaS的共有基因为7 362个,涉及44个功能条目和122条通路;二者的特有基因分别为195和278个.AaM vs AaS比较组含有977个上调基因和687个下调基因,分别涉及32和32个功能条目;此外,上述DEG还涉及糖酵解/糖异生、次级代谢物的生物合成等113条通路.基于比较转录组分析发现球囊菌菌丝和孢子的共有基因、特有基因和DEG可能通过调节细胞活动、代谢进程、内吞作用以及MAPK信号通路等关键途径参与球囊菌的生长、发育和生殖过程.
英文摘要
    In order to explore the correlation among the common genes, specific genes and differentially expressed genes (DEGs) of mycelium (AaM) and spore (AaS) samples of Ascosphaera apis cultured in lab condition with the fungal growth, development, reproduction and pathogenicity, comparative transcriptome analysis of AaM and AaS was performed using RNA-seq technology and bioinformatic methods, followed by RT-qPCR to confirm the reliability of the sequencing data. The results showed that there were 7,362 genes shared by AaM and AaS, involving 44 functional terms and 122 pathways. The specific genes of AaM and AaS were 195 and 278, respectively. In AaM vs AaS comparison group, 977 up-regulated genes and 687 down-regulated genes were discovered, which were associated with 32 and 32 functional terms, respectively. Additionally, the DEGs mentioned above were related to 113 pathways such as glycolysis/gluconeogenesis and biosynthesis of secondary metabolites. Based on comparative transcriptome analysis, it’s revealed that the common genes, specific genes and DEGs may participate in the growth, development and reproduction process of A.apis mycelium and spore through regulating several pivotal pathways such as cell activity, metabolic process, endocytosis and MAPK signaling pathway.

您是第 3872690 位访问者

版权所有 @ 2007《四川大学学报 (自然科学版)》编辑部
地址: 四川省成都市武侯区四川大学望江校区文科楼330至342室  邮编: 610064
电话: (028)85410393  传真: (028)85410393  E-mail: scdx@scu.edu.cn
本系统由北京勤云科技发展有限公司设计