过表达本氏烟基因NbPAP2对转基因植物生长发育的影响

Overexpression of purple phosphatase gene NbPAP2 in tobacco promotes the growth and development of transgenic plants

摘要:

为探究紫色酸性磷酸酶基因在本氏烟草中所发挥的生物学功能，该研究利用生物信息学软件发现本氏烟NbPAP2与拟南芥AtPAP2的氨基酸同源性达到64.2%,其具有特征性的信号肽和靶向叶绿体的C端疏水基序以及双核金属离子中心. qRT-PCR结果显示NbPAP2在植物中组成性表达，花瓣中表达量最高，测定叶片气孔导度平均提高30.5%、净光合速率平均提高23%，然而蒽酮比色法测定糖平均含量下降21%.因此，NbPAP2通过介导碳代谢促进本氏烟草花期提前、生物量提高、种子体积增大等.

Abstract:

In order to explore the biological function of the purple acid phosphatase gene in tobacco, this study used bioinformatics software to find that the amino acid homology of NbPAP2 of tobacco and AtPAP2 of arabidopsis reached 64.2%, which is characteristic Signal peptide and C-terminal hydrophobic motif targeting chloroplast and binuclear metal ion center. The results of qRT-PCR showed that NbPAP2 was constitutively expressed in plants, with the highest expression in petals. The leaf stomatal conductance was increased by an average of 30.5%, and the net photosynthetic rate was increased by an average of 23%. However, the sucrose content decreased by 21%. Therefore, NbPAP2 promotes early flowering stage, biomass increasing, and increasing of tobacco seed volume by mediating carbon metabolism.