引用本文格式: 徐树涛,彭锐,邹方东. Napabucasin对结直肠癌细胞增殖及迁移的影响 [J]. 四川大学学报: 自然科学版, 2020, 57: 1193~1197.
 
Napabucasin对结直肠癌细胞增殖及迁移的影响
Effects of Napabucasin on the proliferation and migration of colorectal cancer cells
摘要点击 143  全文点击 85  投稿时间:2020-05-22  修订日期:2020-07-05
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DOI编号   
中文关键词   结直肠癌  STAT3  Napabucasin  细胞迁移
英文关键词   Colorectal cancer  STAT3  Napabucasin  Cell migration
基金项目   国家自然科学基金(81672942)
作者单位E-mail
徐树涛 四川大学生命科学学院 xushut@qq.com 
彭锐 四川大学生命科学学院  
邹方东 四川大学生命科学学院  
Author NameAffiliationE-mail
XU Shu-Tao College of Life Sciences, Sichuan University xushut@qq.com 
PENG Rui College of Life Sciences, Sichuan University  
ZOU Fang-Dong College of Life Sciences, Sichuan University  
中文摘要
    为了探究新型小分子抑制剂Napabucasin对结直肠癌细胞增殖以及迁移的影响. 首先通过分子模拟对接分析了Napabucasin与STAT3蛋白的互作机制. 然后利用克隆形成实验、细胞划痕实验等方法在多种结直肠癌细胞系中证明了Napabucasin能够显著抑制结直肠癌细胞的集落形成能力以及迁移能力. 进而使用Napabucasin与Wnt信号通路激活剂Wnt agonist 1共处理结直肠癌细胞HCT116,结合蛋白质印迹实验发现,Wnt信号通路介导了Napabucasin对结直肠癌细胞的迁移以及增殖的抑制过程. 研究结果显示,Napabucasin能够在体外抑制结直肠癌细胞的增殖能力以及迁移能力,并且Wnt信号通路参与介导了这一抑制过程.
英文摘要
    This study aims to explore the effect of the novel small molecule inhibitor Napabucasin on the proliferation and migration of colorectal cancer cells. Firstly, the interaction mechanism between Napabucasin and STAT3 was analyzed by molecular docking. Then, it was proved that Napabucasin can significantly inhibit the colony formation and migration ability of colorectal cancer cells using clone formation experiments and wound healing assay. Napabucasin and Wnt agonist 1 were then used to co treat the HCT116 cells, combined with Western blot experiments, it was found that the Wnt signaling pathway mediates the inhibition of the migration and proliferation of colorectal cancer cells by Napabucasin. The research results show that Napabucasin can inhibit the proliferation and migration ability of colorectal cancer cells in vitro, and the Wnt signaling pathway is involved in mediating this inhibition process.

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