竹叶花椒TPS基因家族全基因组分析

Genome-wide analysis of TPS gene family of Zanthoxylum armatum

摘要:

为了探究香料植物TPS基因家族的潜在功能，本研究以香料植物竹叶花椒(Zanthoxylum armatum DC.)为材料，利用生物信息学方法对其萜类合成酶基因家族的理化性质、基因重复类型、亚细胞定位、进化关系、染色体定位与共线性、基因结构和基因表达进行了系统地分析. 我们在竹叶花椒全基因组中共鉴定到53个TPS基因家族成员，它们编码蛋白氨基酸长度为173~859 aa，分子量为20.21~98.44 kDa，等电点范围在4.87~9.10，主要定位于细胞质和叶绿体当中. 进化分析显示，ZaTPSs可以划分为6个亚家族，TPS-a和TPS-b亚家族的成员最多. 其中48个成员都包含TPS家族的保守基序motif1. 染色体定位与共线性分析显示，53个成员在11条染色体上不均匀分布，存在4处串联重复，共线性分析证明竹叶花椒与其同科物种甜橙［Citrus sinensis (L.) Osbeck］ 的TPS基因有更近的基因进化关系. 基因表达差异分析显示，ZaTPSs有一定的组织表达特异性，在幼花中表达量最高，其次是果皮. 这些研究结果表明TPS基因家族在竹叶花椒果皮挥发油的合成和防御等方面发挥了重要作用.

Abstract:

In order to explore the potential function of the TPS gene family in perfume plant, a comprehensive investigation of the TPS gene family was conducted in the well-known perfume plant Zanthoxylum armatum, and the basic physical and chemical properties, gene duplication type, subcellular localization, evolutionary relationship, chromosome localization and collinear analysis, gene structure and gene expression pattern were analyzed. A total of 53 ZaTPS genes were identified in the whole genome of Z. armatum. The amino acid length of their proteins ranges from 173 to 859 aa, and the molecular weight ranges from 20.21 to 98.44 kDa. The isoelectric point ranges from 4.87 to 9.10, and the subcellular localizations are mainly in the cytoplasm and chloroplast. Phylogenetic analysis showed that ZaTPSs could be divided into six subfamilies, and the members of TPS-a and TPS-b subfamilies were the majority ones. There are 48 ZaTPSs containing motif1 that are conserved in the TPS proteins. Chromosomal localization and collinear analysis showed that 53 ZaTPSs were unevenly distributed on 11 chromosomes, and there were four pairs of tandem repeats, and the collinear analysis revealed that TPS genes in Z. armatum and Citrus sinensis from the same family have closer evolutionary relationships. The gene differential expression analysis based on transcriptome datasets showed that ZaTPSs had tissuespecific expression patterns, with the highest expression level was found in young flowers, followed by husks. These results indicate that ZaTPSs played an important role in the synthesis of husks volatile oil and defense.