拟南芥AtHHR2基因的功能初步研究

Primary Analysis of the Function of AtHHR2 in Arabidopsis thaliana Primary Analysis of the Function of AtHHR2 in Arabidopsis thaliana

摘要:

拟南芥Highly Homologous RING domain 2基因（At5g43200）编码一个含有RING finger结构域的E3连接酶。根据基因芯片表达谱推测该基因可能在参与高盐胁迫响应等方面起作用。为了研究该基因在盐胁迫逆境的功能，通过DNA及RNA水平鉴定了该基因的T –DNA插入突变体athhr2，并通过根瘤农杆菌介导法进行遗传转化，筛选获得了athhr2/ATHHR2互补转基因株系。对突变体、互补转基因株系及野生型进行盐胁迫处理，对其萌发率、脯氨酸及叶绿素水平进行检测，发现盐处理后缺失突变体的萌发率降低，脯氨酸和叶绿素含量低于野生型，互补株系的萌发率、脯氨酸和叶绿素含量均高于野生型。以上结果证明AtHHR2基因在拟南芥的盐胁迫响应中起正调控作用，为深入分析该基因的功能打下基础。

Abstract:

Highly Homologous RING domain 2（At5g43200）encodes a RING finger E3 ligase. According to publicaly available microarray analysis, this gene may be involved in salt stress response. To study the fuction of At5g43200 in response to salt stress, the T-DNA insertion mutant athhr2 was obtained, and 3 homozugous complementary transgenic lines athhr2/ATHHR2 were obtained. The mutants, complementary transgenic lines and wild-type of Arabidopsis were used for salt stress experiment. Germination Rates and resistance - related physiological indexes of all the plants were measured, including proline content and chlorophyll content. Under salt treatment, the germination rate, relative proline content and chlorophyll content in deficient mutants are lower than the wild-type, and those of complementary transgenic plants are all higher than the wild-type. These results suggested that AtHHR2 gene played a positive regulatory role in Arabidopsis thaliana response to salt stress, our study laid foundation for further analysis on the gene’s function．