苦荞蛋白磷酸酶2C家族的鉴定及表达分析

Genome-wide identification and expression analysis of protein phosphatase 2C family in Tartary buckwheat

摘要:

为了研究苦荞蛋白磷酸酶2C（PP2C）家族的成员和分类，及后续探究其在苦荞生长发育中的功能，本文利用生物信息学方法对苦荞PP2C家族进行鉴定、分类，并对其基因结构、保守基序、分子进化等进行分析. 结果表明，苦荞PP2C家族有 81个成员，划分为AK的11个亚族，并且在同亚族中序列特征相似，而不同亚族间序列特征有一定差异；苦荞PP2C家族有14次基因重复事件. 此外，qRTPCR分析结果表明其A亚族基因在苦荞根、茎、叶、花、果中均有表达，除FtPP2C44外的8个基因在苦荞花、果中表达量较高；在苦荞幼苗中，除FtPP2C08外的8个基因均受ABA诱导表达量上调. 上述结果揭示了苦荞PP2C家族的成员组成、序列特征、扩增和其A亚族基因的组织表达模式及受ABA诱导表达情况.

Abstract:

In order to study the members and classification of protein phosphatase 2C (PP2C) family in Tartary buckwheat and to explore its function in the growth and development of Tartary buckwheat, this study used bioinformatics methods to identify and classify the PP2C genes of Tartary buckwheat (FtPP2C), and its genes structure, conservative motifs, and molecular evolution were analyzed. The results revealed that 81 FtPP2C genes divided into 11 subgroups (A-K), and the sequence characteristics in the same subgroups were similar, but there were certain differences in sequence characteristics among different subgroups. The results also revealed that FtPP2C genes had 14 gene duplication events. In addition, the expression patterns of FtPP2C clade A genes were analyzed by qRT-PCR, and the results revealed that all FtPP2C clade A genes were expressed in five organs (roots, stems, leaves, flowers, and fruits), among them, 8 genes except FtPP2C44 were highly expressed in flowers and fruits; in seedlings, all 8 genes except FtPP2C08 were up-regulated after ABA treatment. The above results revealed that the composition, sequence characteristics, and amplification of the FtPP2C genes. These results also revealed the tissue expression pattern and the expression induced by ABA of FtPP2C clade A genes.