拟南芥钙依赖蛋白激酶CPK11与ABA响应元件结合因子ABF4相互作用的研究

Research on the interaction between CPK11 and ABF4

摘要:

为研究钙依赖蛋白激酶CPK11参与ABA信号通路的方式,本文利用体外酵母双杂实验（Y2H）以及体内双分子荧光互补实验(BiFC)分析CPK11与ABA响应元件结合因子(ABA-responsive element binding factors,ABFs)ABF4之间的关系.酵母双杂交实验表明CPK11与ABF4存在体外相互作用,双分子荧光互补实验表明CPK11与ABF4存在体内相互作用.以上实验共同证明CPK11与ABF4存在直接相互作用.作为CPK11的同源蛋白CPK4与转录因子ABF4在植物体内也存在相互作用.以上实验表明CPK11及其同源蛋白CPK4可能通过与转录因子ABF4相互作用从而参与钙离子介导的ABA信号通路.

Abstract:

To investigate the way that the calcium-dependent protein kinase CPK11 involved in regulating ABA signal transduction, Yeast two hybrid (Y2H) assay and Bimolecular fluorescence complementation (BiFC) assay were used to analyze the relationship between CPK11 and ABA-responsisve element binding factors ABF4. Y2H assay showed that CPK11 and ABF4 had an interaction in vitro, and BIFC assay showed that CPK11 and ABF4 had an interaction in vivo. The above experiments together proved that there was a direct interaction between CPK11 and ABF4. As a homologous protein of CPK11, CPK4 also interacted with transcription factor ABF4 in plants. In summary, these results indicated that CPK11 and its homologous protein CPK4 may participate in calcium-mediated ABA signaling pathway by interacting with transcription factor ABF4.