Human elongation factor 1A1(eEF1A1) promotes drug tolerance in lung cancer cell line

Abstract:

Human translational elongation factor 1A （eEF1A）is a multi-functional protein besides its roles in protein translation system. The protein expressional level of eEF1A is related to cell viability in oxidative stress and apoptotic process. Based on the knowledge of multifunctional roles of eEF1A and its relation to cell viability, we studied the possible effect of the isoform eEF1A1 on the drug resistance in cancer cell lines. The eukaryotic expression vector containing eEF1A1 sequence was constructed and was transiently transfected into human lung cancer cell line H1299. After verifying the expression of FLAG -eEF1A1, MTT assay was utilized to measure the cell viability of transfected H1299 after treatment by two common anti-cancer drugs. H1299 transfected with eEF1A1 showed enhanced drug tolerance to both taxol and adriamycin. Lung cancer cell highly expressing EEF1A1 behave more tolerance to antineoplastic drugs.