Molecular Cloning and Expression Patterns of LcAO from Lycium Chinens

Abstract:

A ascorbate oxidase (AO) gene from Lycium Chinense was cloned with homologous method. The full-length cDNA of Lycium Chinense AO which was named LcAO (Genbank: KP712033) was 1737 bp，and contained a complete open reading frame (ORF) of 578 amino acid residues. Homology analysis indicated that the deduced LcAO protein was highly 90% to AO proteins from Solanum lycopersicum. The results from Real-Time PCR indicated that the expression of LcAO gene was the strongest in the flower and friut, and the least in mature-leaf of Lycium Chinense.