Screening of Phospholipase D producing Strains and its transphosphatidylation activity

Abstract:

Using soybean Phospholipids as substrates，one strain which expressed Phospholipase was isolated from soil near oil mill ，the Phospholipase produce by SNUPLD-6 was identified which contain Phospholipase D . According to its mor -phological characteristics and ITS sequence , The strain SNUPLD-6 was identified as Geotrichum candidum. study on the Conditions for the growth of the bacteria and the PLD reaction conditions which expressed by SNUPLD-6. The results showed that its optimum growthtemperature is 30℃ .its optimum growth pH value is 6.0，its optimum carbon source is glucose，its optimum nitrogen source for the beef extract and peptone 50% each. the optimal fermentation time of phospholipase D producing is 3d, its optimum transphosphatidylation-temperature is 32℃. its optimum transphosphatidylation pH value is 6.0,its optimum reaction buffer for pH=5.5 0.02M HAc -NaAC buffer, its Optimal metalion activator as Ca2 +, Zn2 +.