The Phylogenetic Analysis of Foot-and-mouth Disease Virus (FMDV) Serotype A Based on VP1 Gene

Abstract:

The samples suspected infection of FMDV were identified by RT-PCR method based on VP1 gene. One of the positive samples was sequenced and applied to homologous analysis with other reference sequences, to construct phylogenetic tree of VP1 gene. The results showed that the isolated VP1 belonged to serotype A, Asia topological type, and Sea-97 lineage. It has a high sequence similarity (99.1%) to A/GDMM/CHA/2013, a Chinese A type FMDV isolated in 2013, whereas has a low sequence similarity to the vaccine strain AF/72 (79.6%). The low sequence simi-larity between vaccine strain AF/72 and the A-type FMDV viruses reported recently indicated that AF/72 vaccine strain might only provide limited protection and the vaccine strain should be made up to date as soon as possible.