The analysis of BnTR1 enhances a variety of resistance in Nicotiana benthamiana

Abstract:

Abstract: Owing to the global climate change, frequent drought and salt stress have happened to most plants. As a result, it has become a hot issue in agricultural field to research in tolerance mechanisms of plants and to cultivate new plant materials that can tolerate salt and drought. Derived from rape, BnTR1 is an E3 ligase located in the membrane, and its expression can be induced by heat stress,and then gives the heat resistance to plants. BnTR1 was transformed into Nicotiana benthamiana to study the growth of transgenic Nicotiana benthamiana under drought and salt stress.What’s more, compared with the control, this transgenic plant shows a better resistance to mannitol and PEG, especially in growth status and accumulation of biomass under PEG stress. After 20 days under drought treatment, plants were rewatered and then cultivated for 2 weeks, and all transgenic Nicotiana benthamiana were recovered from wilting, and all wild type Nicotiana benthamiana died. These results indicated that Nicotiana benthamiana can be resistant to drought with BnTR1. When leaves were treated with hydrosaline solution, transgenic Nicotiana benthamiana could maintain green even as salt concentration was above 600mM, while control plants became albino. This result suggested that chloroplast and chlorophyll in transgenic Nicotiana benthamiana can be more stable under salt stress, and transgenic Nicotiana benthamiana can be more resistant to salt stress. After heat shock, the expression of HSP90 and HSP70 in transgenic Nicotiana benthamiana and controls were all up-regulated, and there were no significant difference in increment between transgenic plants and controls. The expression of HSF30 and sHSP17.6 were also induced which can’t be detected in normal condition, HSF30 increasing 50% and sHSP17.6 increasing 30%. These researches illustrated that Nicotiana benthamiana can survive in many stressful conditions when transformed with BnTR1. This experiment laid a good foundation for the study of BnTR1, and also provided an experimental proof for its application.