TAp73α-mediated cisplatin sensitivity was suppressed by miR-330 in colorectal cancer cell HCT116

Abstract:

The p53 family member p73 is a transcription factor involved in the regulation of cell cycle, apoptosis and cancer cell drug-resistant. Here we reported that knockdown of p73 sensitized HCT116 colorectal cancer cells to cisplatin treatment independent of p53. Hsa-miR-330 (miR-330) was identified to be able to directly repress the expression of TAp73α. Overexpression of miR-330 decreased the protein levels of endogenous TAp73α and phenocopied the effect of p73 knockdown. Restoration of TAp73α eliminated miR-330-induced chemosensitivity toward cisplatin. Our results demonstrated a novel function of TAp73α to impair cisplatin sensitivity in colorectal cancer cells which can be repressed by miR-330, thus provided an effective strategy for therapeutic treatment of cisplatin-resistant cancer cells.

Key words:P73; MiR-330; Apoptosis; Cisplatin; Colorectal cancer