Expression, purification and identification of wound healing function of Interleukin-24 fusion protein

Abstract:

Interleukin-10 family is one of the important cytokine that can cause psoriasis related diseases, and interleukin-24 is relatively important in cascade effect. As a new discovery member of interleukin-10 family, we aim to study the role of interleukin-24 in psoriasis and pro-inflammatory response. In this study, we use biosynthetic gene interleukin 24 mice (mIL-24) to built mIL-24-Fc fusion protein. Then the recombinant plasmid was transfected into CHO cell. After serum-free culture in CHO, we purified mIL-24-Fc fusion protein by protein A affinity chromatography and confirmed by AP binding assay. The result showed that after intradermal injected mice, a large number of keratinocytes showed two to three proliferation and in wound-healing process, mIL-24-Fc can promote epidermal hyperplasia and accelerate wound healing. These findings mean that interleukin424 plays an important role in the inflammatory response, and medical value.