Primary Analysis of the Function of Arabidopsis thaliana RING finger ABRv1

Abstract:

ABscisic acid responsive RING-v protein 1(ABRv1) in Arabidopsis thaliana encodes a E3 ligase with a RING finger structure domain. In an effort to analyze the function of ABRv1 in the plant, we performed abscisic acid (ABA) stress experiments on the wild-type, the ARBv1-overexpressing lines and the T-DNA insertion mutants abrv1. The overexpressing lines were acquired through transfection by Agrobacterium with ABRv1 embedded plasmids (pBI121- ABRv1). The mutants obtained were identified by DNA and RNA analysis. With ABA treatment, the mutants presented a lower germination rate than the wild-type while the overexpressing lines showed a higher rate. Little stomatal closure was observed in the the mutants whereas obvious closure was discovered in the overexpresssing lines. The wild-type showed a closure status in between. Therefore, our phenotypic analysis revealed that ABRv1 might participate in the plant response to ABA as a negative regulator.