Studies on the mechanism of Stellera chamaejasme L. to Locusta migratoria manilensis (Meyen)

Abstract:

Using silica gel column to separate the ethyl acetate extract of Stellera chamaejasme L. we can get I, II, III sections. The activity of Na+-K+-ATPase、Ca2+/Mg2+-ATPase、acetylcholinesterase in insects were determined in vivo. The results showed that I section from Stellera chamaejasme L. had the strongest poison effect on Locusta migratoria manilensis (Meyen). Under the concentration of 10 mg/mL, the corrected mortality was 92.5% in 72 hour. And the poison effect there was persistence. The I section from Stellera chamaejasme L. also had certain inhibition on activity of Na+-K+-ATPase、Ca2+/Mg2+-ATPase、acetylcholinesterase. The inhibiton rate of Na+-K+-ATPase was the highest among the three kinds of enzymes. There was a well correlation between the toxic action of the active ingredients from Stellera chamaejasme L. to Locusta migratoria manilensis (Meyen) and the inhibition on activity of Na+-K+-ATPase in Locusta migratoria manilensis (Meyen). Na+-K+-ATPase is likely the target of the active ingredients from Stellera chamaejasme L. against Locusta migratoria manilensis (Meyen).