Preparation of monoclonal antibody against canine distemper virus of rhesus monkey using gene vaccine

Abstract:

To prepare the monoclonal antibody of Canine distemper virus which infect rhrsus monkey, the H gene of the virus was amplified by RT-PCR and cloned into pVAX. The DNA vaccine pVAX-H was injected into Balb/c mice and induced a strong immunity. The spleen cells were fused with SP2/0 cells and 5 hybridoma strains secreting monoclonal antibodies against CDV H gene were achieved. The five different McAbs didn’t react with CPV and MV but could neutralize CDV in vitro. Two of them had high neutralization antibody above 1:256. The result import that the CDV H gene vaccine can be used to prepare monoclonal antibody.