The research on interaction between the Protein kinase CARK7 and the ABA receptor RCAR12 in Arabidopsis

Abstract:

The ABA receptor RCARs play a key role in ABA Signal transduction process, and research on interaction proteins of RCARs has great significance in further understanding the molecular mechanism of plants in adverse environment. The yeast two-hybrid assay and GST-pull down were used to study the interaction between CARK7 and RCAR12. The results showed that there is a significant interaction between CARK7 and RCAR12 in vitro. Then, the bimolecular fluorescence complementation (BiFC) was used to confirm the interaction between CARK7 and RCAR12 in vivo. The BiFC assay indicated that CARK7 was associated with RCAR12 in tobacco epidermal cells. Taken together, these data suggested that CARK7 and RCAR12 closely interact with each other, and illuminating the regulation of the ABA signaling transduction.