An Interaction of ABA Receptor RCAR12 with CARK3 in Arabidopsis thaliana

Abstract:

The phytohormone abscisic acid (ABA) is crucial for plant development and response to abiotic stress, including drought. ABA perceived by the ABA receptors, PYR/PYL/RCAR, which bound to ABA, and then recruit Protein phosphatase 2C (PP2C) to inhibit the PP2C acticity, thereby activating SNF1-RELATED PROTEIN KINASE2 (SnRK2s). Here, CARK3 and RCAR12 interact in yeast two-hybrid system and GST-pull down in vitro. Bimolecular fluorescence complementation (BiFC) was used to illuminate the interaction between CARK3 and RCAR12 in Nicotiana benthamiana in vivo. We could confer that CARK3 indeed interacts with RCAR12 in present study and the phosphorylation modulates the physiological response of the core ABA signaling pathway. Our results provide the foundation for further understanding of the regulatory network of phosphorylation of ABA signaling pathways core components.