Interaction study between CARK4 and RCAR12 in Arabidopsis thaliana

Abstract:

Abscisic acid receptors play a pivotal role in regulation of environmental stress, its network is fundamental to elicit ABA pathway. In this research, Yeast two- hybrid system (Y2H), Pull down and Bimolecular fluorescence complementation (BiFC) assays were used to study the interaction between CARK4 and RCAR12. The result from Y2H showed an interaction of CARK4ΔN with RCAR12. The same from Pull down were confirmed in vitro. BiFC result indicated that CARK4 interacted with RCAR12 in Nicotiana benthamiana. Taken together, we can conclude that the interact on of CARK4 with RCAR12 is confirmed in vitro and vivo.