Transcriptome analysis on stem development of a Brassica napus L. dwarf mutant

Abstract:

'NDF-1' is a Brassica napus L. dwarf mutant, which was derived from physical and chemical mutagenesis of a high stemmed line '3529 '，possesses a stable dwarf phenotype of approximately one third the height of high stalk parent '3529'. It is an excellent resource dwarf Brassica napus L. RNA-Seq based transcriptome analysis on stem elongation of the dwarf mutant from Bolting Stage (BS) and Florescence Stage (FS), which was conducted to provide a reference for the genome level in order to understand the mechanism of rapeseed stem development. A total of 455 million clean reads were obtained from two different stages include Bolting Stage (BS) and Florescence Stage (FS). Then 2,147 differentially expressed genes (DEGs) were found which detected between 'NDF-1' and '3529' after assembled them. Furthermore, twenty differentially expressed genes were selected for RT-PCR validation. With the GO and KEGG pathway analysis, 'NDF-1' stems elongation most regulated by the gibberellin、auxin and brassinosteroids, cell wall formation or cells Elongation is not normal but also play an important role in 'NDF-1' dwarf. Screening genes associated with plant dwarfing traits from DEGs, which provide a reference for further use of these genes on traits improvement and dwarfing mechanism research.