The metabolism of H2S is inbibited by saccharomyces cerevisiae SCH9 gene deletion

Abstract:

Hydrogen sulfide (H2S) is the third endogenous signaling molecule after nitric oxide and carbon monoxide. Attention towards H2S has been increasing due to its significant role in the cardiovascular and nervous systems. Saccharomyces cerevisiae Sch9 is homologous to human S6K1, which regulate cell cycle as well as life span. The SCH9 deletion and limited increase in the intracellular H2S can prolong the lifespan of animals. They both are also involved in the mechanism of caloric restriction to extend life.In order to study whether SCH9 deletion can affect the metabolism of H2S in yeast . GYY4137 and NaHS were used as H2S donors and lead acetate paper was employed for testing H2S in SCH9 deletion strains RCD398, RCD399 and TS120-2d.Results indicated that synthesis of H2S is negatively regulated by SCH9 deletion strains with or without caloric restriction. Similarly, catabolism of H2S were down regulated.Thus we speculate that SCH9 deletion decreased synthesis and catabolism of H2S in yeast, which laid a theoretical foundation for further study of H2S metabolism.

Key words:Sch9; H2S; metabolism; saccharomyces cerevisiae