Analysis of the interaction between AtHHR2 and DREB2C in Arabidopsis thaliana

Abstract:

A previous report has revealed that AtHHR2 (At5g43200) played a positive regulatory role in Arabidopsis thaliana in salt stress, and its homologous gene AtHHR3 has interaction with DREB2C gene in yeast twohybrid test. In this study, in vitro and in vivo protein interaction assays and biochemical methods were used to further explore the functionality of AtHHR2. And Pulldown result validated the interaction between AtHHR2 and DREB2C in vitro. And the ubiquitination analysis demonstrated that AtHHR2 acted as an E3 ligase of DREB2C, further confirming the interaction between AtHHR2 and DREB2C. Furthermore, bimolecular fluorescence complementation assay results showed that AtHHR2 did have interaction with DREB2C in vivo. In summary, the AtHHR2 interacts with DREB2C and acts as an E3 ligase.