Establishment of a direct multiplex PCR detection system of Salmonella pullorum

Abstract:

In this paper, we screened out three pairs of specific detection primers of Salmonella pullorum, ipaJ417, traJ-387, and traJ-476, by the blast analysis of the coding genes ipaJ and traJ which coded the invasion plasmid antigen protein J and plasmid co-transfer regulatory factor in Salmonella pullorum virulence plasmid Pspuv. And these PCR primers were designed in special regions and identificated in the different species of Salmonella and common intestinal pathogens. And a common reaction buffer of two kinds of DNA polymerase for the direct triple PCR system was also established (invA-211/traJ-387/16S-495). This direct triple PCR system can be used for fast and accurate identification of Salmonella pullorum.