Detection of serum cytokines of autoimmune liver disease patients by high throughput cytokine antibody microarray

Abstract:

The antibody microarray technique was used to detect the expression of 20 kinds of helper T cellassociated inflammatory cytokines in serum of 53 patients suffered with autoimmune liver disease (AILD). Combining with the clinical biochemical data of liver function to analyze the expression characteristics and differences in inflammatory factors in serum of patients with autoimmune liver disease (AILD). The results showed that the levels of IL4 and TGFβ1 in serum of patients with AIH-PBC OS were significantly higher than those of PBC (P<0.05), while the levels of IL-4 in serum of patients with AIH-PBC OS were significantly higher than that of healthy controls (P<0.05). The levels of IL-4 and TGFβ1 in serum of PBC patients were significantly lower than those of healthy controls (P<0.05).The level of TGFβ1 in serum of PBC patients was negatively correlated with ALT (r=-0.6301, P=0.0135) and AST (r=-0.7443, P=0.0030). Conclusion: the expression levels of IL-4 and TGFβ1 in the blood serum of AIH-PBC OS, PBC patients and the expression level of TGFβ1 was of favorable correlation with the liver function indexes of AILD patients，which indicates it is potential application in AILD screening, diagnosis, and prognosis.