The research on interaction between Arabidopsis Calcium-dependent Kinanse CPK12 and bZIP transcription factor ABF4

Abstract:

In order to analyze the sequence similarity of the Arabidopsis calciumdependent protein kinase CPK12 in different species and the function of CPK12 in the ABA signaling, this study carried on the amino acid sequence alignment of CPK12 among different species, and focused on the direct interaction between CPK12 and ABF4 in vivo and in vitro. The amino acid sequence alignment was conducted to analyze CPK12 homology among the Arabidopsis thaliana, Brassica napus, Capsella rubella, etc. In addition, CPK12 and ABF4 were co-transfected through the yeast twohybrid system. The result showed that yeasts could grow normally on SD (His/Ade Leu/Trp) agar medium, indicating that CPK12 interacted with ABF4 in vitro. In the bimolecular fluorescence complementary assay, the obvious yellow fluorescence signal could be observed in the nucleus when CPK12 and ABF4 were co-transfected into tobacco. The result also demonstrated that CPK12 interacted with ABF4 in vivo. All these provided direct evidence that CPK12 was involved in the ABA signaling.