Analysis of the interaction between E3 ubiquitin ligase AtARRE and ABI5 in Arabidopsis thaliana

Abstract:

Based on the study of AtARRE at the gene and protein levels, this paper describes its role with the key transcription factor ABI5 of the ABA signaling pathway. In this study, a protoplast transient expression system was used to explore the subcellular localization of AtARRE protein, and the result demonstrated that AtARRE localized in the nucleus. Subsequently, the interaction between AtARRE and ABI5 was analyzed by yeast two-hybrid and GST-Pull down techniques. Our analysis demonstrated that AtARRE interacted with ABI5 in vitro. At last, analysis by bimolecular fluorescence complementation experiments further demonstrated the interaction between AtARRE and ABI5, indicating that the co-expression of AtARRE and ABI5 had interaction in vitro. These results indicate that AtARRE may be involved in ABI5-mediated plant response to stress.