Analysis of photosynthetic physiological characteristics of loss-of-function of STN7 in rice ( Oyrza sativa)

Abstract:

In this experiment, it was designed to investigate the differences in photosynthetic performance between wildtype (WT) and stn7 mutant. The seed germination rate of two rice was detected. Besides, chlorophyll content was measured by pulse amplitude modulation imaging fluorometer IMAGMINI, SPAD502 chlorophyll meter was used to measured the SPAD value of plants in the seedling stage, and the light response curve of the plants at jointingbooting stage under natural growth was measured by the LI6400XT portable photosynthesis system. The results showed that there was almost no difference in seed germination and SPAD value between WT and stn7. The result of chlorophyll fluorescence parameters indicates that the photosynthetic performance of WT is better than stn7. The result of chlorophyll a/b value and light response curve reveals the ability to use low light in WT is stronger. And the result of the light response curve shows that the photosynthetic rate of stn7 is higher than WT in high light. These results attest that the STN7 kinase is important for the photosynthesis of rice